The study aimed at determining the incidence, nature, and extent of childhood injuries in two suburban and rural communities of Pakistan. The findings of the study are based on a cross-sectional survey of 2,292 children aged 1-8 years. Information was sought retrospectively from the primary caregiver on the occurrence of injury that required formal or informal medical consultation during the past three months. The incidence rate of non-fatal injuries that required care outside home for children aged 1-8 years was 19.7 injuries per 100 person (child)-years of exposure [95% confidence interval (CI) 16.41-23.51]: 26.5 injuries per 100 person (child)-years of exposure (95% CI 21.31-32.63) in the suburban area and 12.1 injuries per 100 person (child)-years of exposure (95% CI 8.68-16.66) in the rural area. The most common non-fatal injuries were falls (10.5 fall injuries per 100 person (child)-years of exposure), bums and scalds (3.5 burn injuries per 100 person (child)-years of exposure), and road traffic injuries (RTIs) (2 RTIs per 100 person (child)-years of exposure). One fatality due to drowning was also reported during the study period. The difference among sex was highly significant (p = 0.03). Boys (60%) were at a higher risk of injuries compared to girls (40%). The data also revealed that 61% of the injuries took place inside the home. The magnitude of childhood injuries in the two communities was significant compared to the findings of the National Health Survey of Pakistan (1990-1994). The fact that the majority (61%) of the injuries occurred inside the home raises many questions in relation to the household hazards and adequacy of safety and child-proofing measures in these households. There is a need to develop community-based interventions, creating awareness about the consequences of childhood injuries and educating families about preventive measures to reduce the incidence of injuries during early and middle childhood.