The development of a new 200-item questionnaire based on the theoretical constructs of the alternative Five-factor model of personality is described. We developed the Zuckerman-Kuhlman-Aluja Personality Questionnaire (ZKA-PQ) from an initial pool of 537 items. Its final version includes 5 factors with 4 facets per factor and 10 items per facet. Internal consistencies were adequate particularly for the factors. The 1 factor confirmatory factor analyses showed satisfactory goodness-of-fit indexes, but not for the 5 factor simple structure. When incorporating the secondary loadings and the correlated error terms, the model fit improved. A multigroup analysis showed gender differences for the factors Sensation Seeking, Neuroticism, Aggressiveness, and Activity for the Spanish-speaking sample but only for Aggressiveness in the English-speaking sample. We assessed the convergent and discriminant validity of the ZKA-PQ by inspecting correlations with shortened versions of the Revised NEO Personality Inventory (Costa & McCrae, 1992) and Temperament and Character Inventory-Revised (Cloninger, 1999) in 2 independent and additional samples. This new instrument may be useful for basic and applied research, including normal personality, psychobiology of personality, personality and clinical disorders, and industrial-organizational psychology.