Background Road Traffic Injury (RTI) is one of the major causes of death, injury and disability worldwide and most of which occur in developing countries like Bangladesh. The main objective of this study is to identify the role of various socio-demographic and economic factors regarding the knowledge and consciousness about RTI at Khulna division in Bangladesh.

Methods Primary data were collected from 200 respondents in Khulna Medical College Hospital and Satkhira Sadar Hospital and several private clinics, generated by interviewing people who had experiences traffic crash in Khulna division, Bangladesh and the Chi-square test and the logistic regression model have been utilized in this study.

Results The results show that, there was a significant association between education (primary to HSC: OR=3.584, 95% CI: 0.907 to 14.155; higher educated: OR=24.070, 95% CI=4.860–119.206); occupation (farmer and labor: OR = 0.528,95% CI = 0.208–1.340; others: OR = 0.263, 95% CI = 0.097–0.713); motorcycle (OR=4.137, 95% CI = 1.229–13.932); proper treatment (OR=4.690, 95% CI = 1.736–12.673); consciousness about RTI (OR=18.394, 95% CI = 6.381–53.025); unskilled driver (OR=8.169, 95% CI=0.96–16.51), unfit vehicles (OR=3.696, 95% CI=1.032–13.234), breaking traffic rules (OR=6.918, 95% CI=2.237–21.397), faulty road and management (OR=3.037, 95% CI=1.125–8.196) with knowledge about traffic rules in Khulna division, Bangladesh.

Conclusion According to the result of the study, by increasing knowledge and awareness about traffic rules among people through education and awareness program, imposing strict traffic rules, should not give license to unskilled drivers and unfit vehicles, reconstruction of the road and by building proper road management RTI can be reduced.